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Ahsa1 Antibody

AHA1 antibody

Product Specifications

Product Name Alternative

Rat anti-Aha1 Antibody, Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody

UniProt

Q8BK64

Reactivity

Human

Immunogen

This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.

Target

Ahsa1

Clonality

Monoclonal

Clone

25F2.D9

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000, IHC: 5-10 μg/mL, WB: 1:1,000

Purity

This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to Aha1 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_666148.1

Host or Source

Rat

Preservative

Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG2a

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