Stat1 Antibody

Rabbit anti-Stat1 pY701 Antibody, DKFZp686B04100 antibody, ISGF 3 antibody, Signal transducer and activator of transcription 1 91kDa antibody, Signal transducer and activator of transcription 1 alpha/beta antibody, Signal transducer and activator of transcription 1 antibody

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.

This product was Protein A purified from monospecific antiserum. This antibody is specific for mouse Stat1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted. Cross-reactivity with Stat1 from other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.