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Sephs2 Antibody

Selenophosphate Synthetase 2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Selenophosphate Synthetase 2 Antibody, rabbit anti-SPS 2 antibody, Selenide water dikinase 2 antibody, Selenium donor protein 2 antibody, SEPHS2 antibody

UniProt

P97364

Reactivity

Mouse

Immunogen

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.

Target

Sephs2

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

2.3 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000

Purity

This product was purified by Protein A chromatography from monospecific antiserum. This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1. A BLAST analysis was used to suggest cross-reactivity with SPS2 from human sources based on an 84% homology with the immunizing sequence. Cross-reactivity with SPS2 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

14717785

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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