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Nrf1 Antibody

NRF1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody

UniProt

Q9WU00

Reactivity

Human, Mouse

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.

Target

Nrf1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

2.2 mg/mL

Dilution

ELISA: 1:3,000 - 1:10,000, IHC: 2 mg/ml - 5 μg/ml, WB: 1:500 - 1:2,000

Purity

This protein A purified antibody is directed against mouse NRF1. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

13529317

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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