ULP1 Antibody
ULP1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-ULP1 antibody, rabbit anti-ULP-1 antibody, Probable sentrin specific protease antibody, Ubiquitin Like Protease antibody
UniProt
Q02724
Reactivity
Yeast
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
Target
ULP1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
5.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
6325237
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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