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SUMO3 Antibody

SUMO-3 antibody

Product Specifications

Product Name Alternative

Rabbit anti-SUMO-3 Antibody, Small ubiquitin related modifier 3 antibody, SMT3 homolog 1 antibody, SMT3A antibody, SMT3H1 antibody, SUMO 3 antibody, Ubiquitin like protein SMT3A antibody

UniProt

P55854

Reactivity

Human

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.

Target

SUMO3

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

5.0 mg/mL

Dilution

ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates) . Other intrinsic bands are readily detectable at lower dilutions. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kDa in size corresponding to human SUMO-3 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

48928058

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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