SUMO3 Antibody
SUMO-3 antibody
Product Specifications
Product Name Alternative
Rabbit anti-SUMO-3 Antibody, Small ubiquitin related modifier 3 antibody, SMT3 homolog 1 antibody, SMT3A antibody, SMT3H1 antibody, SUMO 3 antibody, Ubiquitin like protein SMT3A antibody
UniProt
P55854
Reactivity
Human
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.
Target
SUMO3
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
5.0 mg/mL
Dilution
ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates) . Other intrinsic bands are readily detectable at lower dilutions. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kDa in size corresponding to human SUMO-3 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
48928058
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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