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URM1 Antibody

Urm1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Urm1 Antibody, C9orf74 antibody, Chromosome 9 open reading frame 74 antibody, MGC2668 antibody, RP11 339B21.4 antibody, Ubiquitin Related Modifier 1 antibody, Ubiquitin related modifier 1 homolog antibody, Urm 1 antibody

UniProt

P40554

Reactivity

Yeast

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Urm1 protein.

Target

URM1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

5.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates) . Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Tested Applications

ELISA, WB

NCBI Accession Number

P40554.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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