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HUB1 Antibody

Hub1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Hub1 Antibody, rabbit anti-Hub 1 Antibody, Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody

UniProt

Q6Q546

Reactivity

Yeast

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.

Target

HUB1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

5.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates) . Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Tested Applications

ELISA, WB

NCBI Accession Number

6324360

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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