ATG8 Antibody

Rabbit anti-APG8 Antibody, APG-8, APG 8, ATG-8, ATG 8, Rabbit Anti-ATG8 Antibody, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5, AUT7, CVT5, YBL078C, YBL0732

Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Store antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Apg8 purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates) . Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast Apg8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.