ATG12 Antibody
APG12 antibody
Product Specifications
Product Name Alternative
Rabbit anti-APG12 antibody, APG-12, APG 12, ATG-12, ATG 12, APG12-like antibody, APG12L antibody, ATG12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog antibody, Autophagy 12 antibody, Autophagy-related protein 12 antibody, HAPG12 antibody
UniProt
P38316
Reactivity
Yeast
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.
Target
ATG12
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
5.0 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates) . Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
Tested Applications
ELISA, WB
NCBI Accession Number
6319694
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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