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ATG12 Antibody

APG12 antibody

Product Specifications

Product Name Alternative

Rabbit anti-APG12 antibody, APG-12, APG 12, ATG-12, ATG 12, APG12-like antibody, APG12L antibody, ATG12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog antibody, Autophagy 12 antibody, Autophagy-related protein 12 antibody, HAPG12 antibody

UniProt

P38316

Reactivity

Yeast

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Target

ATG12

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

5.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000

Purity

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates) . Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

Tested Applications

ELISA, WB

NCBI Accession Number

6319694

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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