ADA Antibody

Anti-Adenosine Deaminase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Adenosine Deaminase antibody has been tested in western blot and is suitable for ELISA and Immunoprecipitation applications. Anti-ADENOSINE DEAMINASE antibody was tested against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and DAB as a substrate at room temperature. A working dilution of 1:8,000 to 1:40,000 is suggested for this product.