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CENP-E Antibody

Mouse monoclonal antibody to CENP-E

Product Specifications

Product Name Alternative

Mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE

UniProt

Q02224

Reactivity

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Target

CENPE

Clonality

Monoclonal

Clone

1H12

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, IF: 1:500 - 1:2,000, IP: 1:200, WB: 1:500 - 1:2,000

Purity

This Protein A purified antibody is directed against human CENP-E protein. The product was purified from tissue culture supernatant by chromatography. This antibody reacts with CENP-E from human cells. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, IP, WB

NCBI Accession Number

CAA78727.1

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG1

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