CENP-E Antibody
Mouse monoclonal antibody to CENP-E
Product Specifications
Product Name Alternative
Mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE
UniProt
Q02224
Reactivity
Human
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
Target
CENPE
Clonality
Monoclonal
Clone
1H12
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:20,000, IF: 1:500 - 1:2,000, IP: 1:200, WB: 1:500 - 1:2,000
Purity
This Protein A purified antibody is directed against human CENP-E protein. The product was purified from tissue culture supernatant by chromatography. This antibody reacts with CENP-E from human cells. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, IP, WB
NCBI Accession Number
CAA78727.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1
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