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INS Antibody

Insulin antibody

Product Specifications

Product Name Alternative

Mouse anti-insulin antibody, ILPR antibody, INS antibody, Insulin A chain antibody, Insulin B chain antibody, Insulin precursor antibody, IRDN antibody, Proinsulin antibody, Proinsulin precursor antibody

UniProt

P01308

Reactivity

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA) .

Target

INS

Clonality

Monoclonal

Clone

2D11.H5

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200

Purity

This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.

Tested Applications

DOT, ELISA, IHC, WB

NCBI Accession Number

P01308.1

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG1

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