INS Antibody
Insulin antibody
Product Specifications
Product Name Alternative
Mouse anti-insulin antibody, ILPR antibody, INS antibody, Insulin A chain antibody, Insulin B chain antibody, Insulin precursor antibody, IRDN antibody, Proinsulin antibody, Proinsulin precursor antibody
UniProt
P01308
Reactivity
Human
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA) .
Target
INS
Clonality
Monoclonal
Clone
2D11.H5
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200
Purity
This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
P01308.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1
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