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TG Antibody

Thyroglobulin antibody

Product Specifications

Product Name Alternative

Mouse anti-Thyroglobulin Antibody, AITD 3 antibody, AITD3 antibody, hTG antibody, TDH3 antibody, Tg antibody, TGN antibody

UniProt

P01266

Reactivity

Canine, Human, Mouse, Rat

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.

Target

TG

Clonality

Monoclonal

Clone

2H11

Conjugation

Unconjugated

Concentration

1.6 mg/ml

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200

Purity

This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded tissue sections can be used without epitope retrieval or enzyme digestion. This antibody is specific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmic localization. Thyroid tissue can be used as a positive control.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

P01266.5

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Isotype

IgG1

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