Hmgn1 Antibody

Mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody

Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation. The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized) . An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid) . IPO-38 does not block the binding of the Ki-67 antibody. Tonsillar tissue is typically used as a positive control.