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Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated

Fab Sheep IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Donkey Fab Anti-Sheep IgG Antibody Peroxidase Conjugation, Donkey Fab Anti-Sheep IgG HRP Conjugated Antibody

Reactivity

Sheep

Immunogen

Sheep IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Epigenetics & Chromatin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:40,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:4,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

ELISA, IHC, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG Fab

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