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Fab Rabbit IgG (H&L) Antibody Biotin Conjugated

Fab Rabbit IgG (H&L) antibody (Biotin)

Product Specifications

Product Name Alternative

Donkey Fab Anti-Rabbit IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Rabbit IgG Antibody Biotin Conjugation

Reactivity

Rabbit

Immunogen

Rabbit IgG whole molecule

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Epigenetics & Chromatin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:75,000 - 1:150,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Fab Anti-Rabbit IgG Biotin Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

ELISA, IHC, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG Fab

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