Fab Rabbit IgG (H&L) Antibody Peroxidase Conjugated
Fab Rabbit IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat Fab Anti-Rabbit IgG Antibody Peroxidase Conjugation, Goat Fab Anti-Rabbit IgG HRP Conjugated Antibody
Reactivity
Rabbit
Immunogen
Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Epigenetics & Chromatin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:60,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Fab Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG Fab
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