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Fab Human IgG (H&L) Antibody Rhodamine Conjugated

Fab Human IgG (H&L) antibody (TRITC)

Product Specifications

Product Name Alternative

Rabbit Fab Anti-Human IgG Antibody Rhodamine Conjugation, Rabbit Fab Anti-Human IgG TRITC Conjugated Antibody

Reactivity

Human

Immunogen

Human IgG whole molecule

Clonality

Polyclonal

Conjugation

TRITC

Field of Research

Epigenetics & Chromatin

Concentration

1.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Fab Anti-Human IgG (H&L) Rhodamine Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.

Tested Applications

FC, FLISA, IF, WB

Host or Source

Rabbit

Preservative

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG Fab

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