Fab Goat IgG (H&L) Antibody Rhodamine Conjugated
Fab Goat IgG (H&L) antibody (TRITC)
Product Specifications
Product Name Alternative
Donkey Fab Anti-Goat Antibody Rhodamine Conjugation, Donkey Fab Anti-Goat TRITC Conjugated Antibody
Reactivity
Goat
Immunogen
Goat IgG whole molecule
Clonality
Polyclonal
Conjugation
TRITC
Field of Research
Epigenetics & Chromatin; Cell Biology
Concentration
1.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Fab Anti-Goat IgG Rhodamine Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.
Tested Applications
DOT, FC, FLISA, IF
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG Fab
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