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F (ab') 2 Sheep IgG (H&L) Antibody Phycoerythrin conjugated

F (ab') 2 Sheep IgG (H&L) antibody (RPE)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 Anti-Sheep IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Sheep IgG Antibody Phycoerythrin Conjugation

Reactivity

Sheep

Immunogen

Sheep IgG whole molecule

Clonality

Polyclonal

Conjugation

RPE

Concentration

0.5 mg/mL

Dilution

FC: 1:100 - 1:250, IF: 1:100 - 1:250

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent) . This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Notes

For research use only.

Applications Notes

F (ab') 2 Anti-Sheep IgG Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Tested Applications

FC, IF

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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