F (ab') 2 Rat IgG Fc Antibody Pre-Adsorbed
Selected Variant
GTR18186349
Size: 1 mg
F (ab') 2 Rat IgG F (c) antibody
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Rat IgG F (c) Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody
Reactivity
Rat
Immunogen
Anti-Rat IgG F (c) was produced by repeated immunization with Rat F (c) fragment in goat.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:3,000, WB: 1:2,000 - 1:5,000
Purity
Anti-Rat F (c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F (c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c), Rat IgG F (ab) or Bovine, Horse and Human Serum Proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Rat IgG F (c) Antibody has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
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