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F (ab') 2 Rat IgG Fc Antibody Pre-Adsorbed

Selected Variant

GTR18186349

Size: 1 mg

F (ab') 2 Rat IgG F (c) antibody

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Rat IgG F (c) Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody

Reactivity

Rat

Immunogen

Anti-Rat IgG F (c) was produced by repeated immunization with Rat F (c) fragment in goat.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:3,000, WB: 1:2,000 - 1:5,000

Purity

Anti-Rat F (c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F (c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c), Rat IgG F (ab) or Bovine, Horse and Human Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

F (ab') 2 Anti-Rat IgG F (c) Antibody has been tested by SDS=PAGE and is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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