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F (ab') 2 Rat IgG (H&L) Antibody Peroxidase Conjugated

Selected Variant

GTR18186342

Size: 500 µg

F (ab') 2 Rat IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Rat IgG Peroxidase Conjugated Antibody, Goat F (ab') 2 Anti-Rat IgG Antibody HRP Conjugation

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

F (ab') 2 Anti-Rat IgG Peroxidase Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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