F (ab') 2 Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated
F (ab') 2 Rabbit IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody alk phos Conjugation
Reactivity
Rabbit
Immunogen
Rabbit IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG F (ab') 2
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