F (ab') 2 Human IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed
Selected Variant
GTR18186194
Size: 500 µg
F (ab') 2 Human IgG F (c) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Human IgG F (c) Peroxidase Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Peroxidase Conjugated Antibody, Goat F (ab') 2 Anti-Human IgG F (c) Fragment Antibody HRP Conjugated
Reactivity
Human
Immunogen
Human IgG F (c) fragment
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
F (ab') 2 Anti-Human IgG Fc was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F (c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c), Human IgG F (ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Human IgG F (c) Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
Price
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