F (ab') 2 Human IgG Antibody Pre-Adsorbed
F (ab') 2 Human IgG (H&L) antibody
Product Specifications
Product Name Alternative
Goat F (ab') 2 anti-Human IgG Antibody, Gt-a-Human F (ab') 2 IgG (H&L), Human F (ab') 2 IgG (H&L) Antibody in goat, Goat IgG (H&L) Secondary Antibody.
Reactivity
Human
Immunogen
Anti-Human IgG was produced by repeated immunization with human IgG whole molecule in goat.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:42,000, IHC: 1:500 - 1:2,000, WB: 1:1,000 - 1:5,000
Purity
F (ab') 2 Anti-Human IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F (c), or Bovine, Horse, Mouse and Rat Serum Proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Human IgG (H&L) Antibody has been tested by ELISA, SDS-PAGE, and dot blot and is suitable for ELISA, immunoblotting, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
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