F (ab') 2 Human (kappa chain) Antibody
F (ab') 2 Human kappa (kappa chain) antibody
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Human κ (kappa chain) Antibody, Goat F (ab') 2 Anti-Human kappa chain Antibody
Reactivity
Human
Immunogen
Anti-Human IgG (kappa chain) was produced by repeated immunization with human IgG kappa light chain in goat.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:25,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) . Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Human IgG (kappa chain) Antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Isotype
IgG F (ab') 2
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