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F (ab') 2 Human (kappa chain) Antibody

F (ab') 2 Human kappa (kappa chain) antibody

Product Specifications

Product Name Alternative

Goat F (ab') 2 Anti-Human κ (kappa chain) Antibody, Goat F (ab') 2 Anti-Human kappa chain Antibody

Reactivity

Human

Immunogen

Anti-Human IgG (kappa chain) was produced by repeated immunization with human IgG kappa light chain in goat.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:25,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) . Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Human IgG (kappa chain) Antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Isotype

IgG F (ab') 2

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