F (ab') 2 Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Selected Variant
GTR18186143
Size: 500 µg
F (ab') 2 Guinea Pig IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Rabbit F (ab') 2 Anti-Guinea Pig IgG Antibody Alkaline Phosphatase Conjugation, Rabbit Fab2 Anti-Guinea Pig IgG alk phos Conjugated Antibody
Reactivity
Guinea pig
Immunogen
Guinea Pig IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Neuroscience
Concentration
1 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:1,000, WB: 1:1,000-1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Guinea pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG F (ab') 2
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