F (ab') 2 Chicken IgG (H&L) Antibody Phycoerythrin conjugated
F (ab') 2 Chicken IgG (H&L) antibody (RPE)
Product Specifications
Product Name Alternative
Rabbit F (ab') 2 Anti-Chicken IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti Chicken Phycoerythrin Conjugated Antibody, Rabbit Fab'2 Anti-Chicken Antibody PE Conjugation, Rabbit F (ab') 2 Anti-Chicken IgY Phycoerythrin
Reactivity
Gallus
Immunogen
Chicken IgG whole molecule
Clonality
Polyclonal
Conjugation
RPE
Concentration
0.5 mg/mL
Dilution
FC: 1:100 - 1:250, IF: 1:100 - 1:250
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent) . This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Notes
For research use only.
Applications Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
FC, IF
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
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