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F (ab') 2 Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated

F (ab') 2 Chicken IgG (H&L) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 Anti-Chicken IgG Alkaline Phosphatase Conjugated Antibody, Rabbit Fab2 Anti Chicken Alkaline Phosphatase Conjugated Antibody, Rabbit Fab'2 Anti-Chicken Antibody ALP Conjugation, Rabbit F (ab') 2 Anti-Chicken IgY Alkaline Phosphatase

Reactivity

Gallus

Immunogen

Chicken IgG whole molecule

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

0.5 mg/mL

Dilution

ELISA: 1:4,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

F (ab') 2 Chicken IgG (H&L) antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG F (ab') 2

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