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F (ab') 2 Cat IgG (H&L) Antibody Peroxidase Conjugated

F (ab') 2 Cat IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 Anti-Cat peroxidase Conjugated Antibody, Rabbit Fab2 Anti-Cat Antibody peroxidase Conjugation, Rabbit Fab'2 Anti-Cat Antibody HRP Conjugation

Reactivity

Feline

Immunogen

Anti-Cat IgG (H&L) was produced by repeated immunization with Cat IgG whole molecule in rabbit.

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Metabolism Research

Concentration

1.0 mg/mL

Dilution

ELISA: 1:100,000, IHC: 1:500 - 1:5,000, WB: 1:5,000 - 1:100,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

F (ab') 2 anti-Cat IgG Peroxidase conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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