F (ab') 2 Bovine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Rabbit F (ab') 2 Anti-Bovine alkaline phosphatase Conjugated Antibody, Rabbit Fab2 Anti-Bovine alkaline phosphatase Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody alk phos Conjugation

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F (c) .

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) ; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0