Donkey IgG (H&L) Antibody Biotin Conjugated

Anti-Donkey IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Donkey IgG and Donkey Serum.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Donkey IgG (H&L) has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2