Sheep IgG (H&L) Antibody Biotin Conjugated
Sheep IgG (H&L) antibody (Biotin)
Product Specifications
Product Name Alternative
Donkey Anti-Sheep IgG biotin Conjugated Antibody, Donkey Anti-Sheep IgG Antibody BAC Conjugation
Reactivity
Sheep
Immunogen
Anti-Sheep IgG whole molecule was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Clonality
Polyclonal
Conjugation
Biotin
Concentration
2.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Sheep IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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