Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Sheep IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Donkey Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody Alk phos Conjugation
Reactivity
Sheep
Immunogen
Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
Purity
Anti-Sheep IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Sheep IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Donkey
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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