Sheep IgG (H&L) Antibody
Sheep IgG (H&L) antibody
Product Specifications
Product Name Alternative
Donkey Anti-Sheep IgG Antibody
Reactivity
Sheep
Immunogen
Sheep IgG whole molecule
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Sheep IgG secondary antibody has been tested by ELISA and is suitable for use in various immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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