Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Sheep IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Rabbit Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody alk phos Conjugation
Reactivity
Sheep
Immunogen
Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in rabbit.
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Sheep IgG (H&L) Alkaline Phosphatase has been tested by ELISA and dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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