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Sheep IgG (H&L) Antibody Pre-Adsorbed

Sheep IgG (H&L) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Sheep IgG pre-adsorbed antibody

Reactivity

Sheep

Immunogen

Sheep IgG whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.4 mg/mL

Dilution

ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Isotype

IgG

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