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Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Rat IgG (H&L) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit Anti-Rat IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Rat IgG Antibody alk phos Conjugation

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1,500 - 1:7,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Rat IgG Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for use in immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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