Rat IgG Antibody Alkaline Phosphatase Conjugated
Rat-IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Rabbit Anti-Rat IgG Antibody alkaline phosphatase Conjugated, Rabbit Anti-Rat IgG Antibody alk phos Conjugation
Reactivity
Rat
Immunogen
Rat IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
Purity
Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti-Rat IgG Secondary Antibody conjugate is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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