Rat IgG F (ab') 2 Antibody Peroxidase Conjugated
Rat IgG F (ab') 2 antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit Anti-Rat IgG F (ab') 2 peroxidase Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 peroxidase Conjugated Antibody, Rabbit Anti-Rat IgG Fab2 Fragment Antibody HRP Conjugation
Reactivity
Rat
Immunogen
Rat IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
HRP
Concentration
2.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F (ab’) 2 and Rat Serum. No reaction was observed against Rat IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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