Suc-Ala-Ala-Phe-AMC

Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays[1][2][3].

Product Specifications

CAS Number

[71973-79-0]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/suc-ala-ala-phe-amc.html

Purity

99.89

Solubility

DMSO : 250 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CC(C(C(O1)=C2)=CC=C2NC([C@@H](NC([C@H](C)NC([C@H](C)NC(CCC(O)=O)=O)=O)=O)CC3=CC=CC=C3)=O)=CC1=O

Molecular Formula

C29H32N4O8

Molecular Weight

564.59

Precautions

H315, H319, H335

References & Citations

[1]Farach HA Jr, et al. Evidence for the involvement of metalloendoproteases in the acrosome reaction in sea urchin sperm. J Biol Chem. 1987 Apr 25;262 (12) :5483-7.|[2]Pinto MR, et al. Chymotrypsin-like enzymes are involved in sperm penetration through the vitelline coat of Ciona intestinalis egg. Mol Reprod Dev. 1990 Aug;26 (4) :319-23.|[3]Mumford RA, et al. A zinc metalloendopeptidase associated with dog pancreatic membranes. J Biol Chem. 1980 Mar 25;255 (6) :2227-30.