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Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Rat IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat Anti-Rat IgG peroxidase Conjugated Antibody, Goat Anti-Rat IgG Antibody HRP Conjugation

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Concentration

2.0 mg/mL

Dilution

ELISA: 1:50,000 - 1:350,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rat IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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