Rat IgA (alpha chain) Antibody Peroxidase Conjugated

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Rat IgA antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2