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Rat IgM (mu chain) Antibody

Rat IgM (mu chain) antibody

Product Specifications

Product Name Alternative

Goat anti-rat IgM

Reactivity

Rat

Immunogen

Rat IgM whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.160 mg/mL

Dilution

ELISA: 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 to 1:10,000

Purity

Anti-RAT IgM (mu chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-RAT IgM (mu chain) (GOAT) Antibody has been tested by ELISA and western blot and is assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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