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Rat IgG Antibody pre-absorbed

RAT IgG (H&L) (Goat) antibody

Product Specifications

Product Name Alternative

Goat Anti Rat IgG Antibody, Goat Anti-Rat IgG Antibody

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10.2mg/mL

Dilution

ELISA: 1:20,000 - 1:110,000, IHC: 1:5,000 - 1:20,000, WB: 1:10,000 - 1:40,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed by ELISA at less than 1% reactivity.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Rat IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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