Rat IgG Antibody pre-absorbed
RAT IgG (H&L) (Goat) antibody
Product Specifications
Product Name Alternative
Goat Anti Rat IgG Antibody, Goat Anti-Rat IgG Antibody
Reactivity
Rat
Immunogen
Rat IgG whole molecule
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
10.2mg/mL
Dilution
ELISA: 1:20,000 - 1:110,000, IHC: 1:5,000 - 1:20,000, WB: 1:10,000 - 1:40,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Mouse Serum Proteins. Specificity was confirmed by ELISA at less than 1% reactivity.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Rat IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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