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Rabbit IgG Fc Antibody Biotin Conjugated

Rabbit IgG F (c) antibody (Biotin)

Product Specifications

Product Name Alternative

Donkey Anti Rabbit IgG F (c) Biotin Conjugated Antibody, Donkey Anti-Rabbit IgG Fc Fragment Antibody Biotin conjugation, Donkey Anti Rabbit IgG Fc Antibody Biotin conjugated

Reactivity

Rabbit

Immunogen

Rabbit IgG F (c) fragment

Clonality

Polyclonal

Conjugation

Biotin

Concentration

2.0 mg/mL

Dilution

ELISA: 1:300,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG, Rabbit IgG F (c) and Rabbit Serum. No reaction was observed against Rabbit IgG F (ab) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG F (c) Biotin antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

ELISA, IHC, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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